Adaptive Nonconforming Finite Element Approximation of Eigenvalue Clusters

This paper analyses an adaptive nonconforming finite element method for eigenvalue clusters of self-adjoint operators and proves optimal convergence rates (with respect to the concept of nonlinear approximation classes) for the approximation of the invariant subspace spanned by the eigenfunctions of the eigenvalue cluster. Applications include eigenvalues of the Laplacian and of the Stokes system

Multiscale Petrov-Galerkin method for high-frequency heterogeneous Helmholtz equations

This paper presents a multiscale Petrov-Galerkin finite element method for time-harmonic acoustic scattering problems with heterogeneous coefficients in the high-frequency regime. We show that the method is pollution free also in the case of heterogeneous media provided that the stability bound of the continuous problem grows at most polynomially with the wave number k. By generalizing classical estimates of Melenk (Ph.D. Thesis 1995) and Hetmaniuk (Commun. Math. Sci. 5, 2007) for homogeneous medium, we show that this assumption of polynomially wave number growth holds true for a particular class of smooth heterogeneous material coefficients. Further, we present numerical examples to verify our stability estimates and implement an example in the wider class of discontinuous coefficients to show computational applicability beyond our limited class of coefficients

Variational Multiscale Stabilization and the Exponential Decay of Fine-scale Correctors

This paper addresses the variational multiscale stabilization of standard finite element methods for linear partial differential equations that exhibit multiscale features. The stabilization is of Petrov-Galerkin type with a standard finite element trial space and a problem-dependent test space based on pre-computed fine-scale correctors. The exponential decay of these correctors and their localisation to local cell problems is rigorously justified. The stabilization eliminates scale-dependent pre-asymptotic effects as they appear for standard finite element discretizations of highly oscillatory problems, e.g., the poor $L^2$ approximation in homogenization problems or the pollution effect in high-frequency acoustic scattering

Impact of infection on proteome-wide glycosylation revealed by distinct signatures for bacterial and viral pathogens

Mechanisms of infection and pathogenesis have predominantly been studied based on differential gene or protein expression. Less is known about posttranslational modifications, which are essential for protein functional diversity. We applied an innovative glycoproteomics method to study the systemic proteome-wide glycosylation in response to infection. The protein site-specific glycosylation was characterized in plasma derived from well-defined controls and patients. We found 3862 unique features, of which we identified 463 distinct intact glycopeptides, that could be mapped to more than 30 different proteins. Statistical analyses were used to derive a glycopeptide signature that enabled significant differentiation between patients with a bacterial or viral infection. Furthermore, supported by a machine learning algorithm, we demonstrated the ability to identify the causative pathogens based on the distinctive host blood plasma glycopeptide signatures. These results illustrate that glycoproteomics holds enormous potential as an innovative approach to improve the interpretation of relevant biological changes in response to infection

Genomic investigations of unexplained acute hepatitis in children

Since its first identification in Scotland, over 1,000 cases of unexplained paediatric hepatitis in children have been reported worldwide, including 278 cases in the UK1. Here we report an investigation of 38 cases, 66 age-matched immunocompetent controls and 21 immunocompromised comparator participants, using a combination of genomic, transcriptomic, proteomic and immunohistochemical methods. We detected high levels of adeno-associated virus 2 (AAV2) DNA in the liver, blood, plasma or stool from 27 of 28 cases. We found low levels of adenovirus (HAdV) and human herpesvirus 6B (HHV-6B) in 23 of 31 and 16 of 23, respectively, of the cases tested. By contrast, AAV2 was infrequently detected and at low titre in the blood or the liver from control children with HAdV, even when profoundly immunosuppressed. AAV2, HAdV and HHV-6 phylogeny excluded the emergence of novel strains in cases. Histological analyses of explanted livers showed enrichment for T cells and B lineage cells. Proteomic comparison of liver tissue from cases and healthy controls identified increased expression of HLA class 2, immunoglobulin variable regions and complement proteins. HAdV and AAV2 proteins were not detected in the livers. Instead, we identified AAV2 DNA complexes reflecting both HAdV-mediated and HHV-6B-mediated replication. We hypothesize that high levels of abnormal AAV2 replication products aided by HAdV and, in severe cases, HHV-6B may have triggered immune-mediated hepatic disease in genetically and immunologically predisposed children

Numerical approximation of planar oblique derivative problems in nondivergence form

A numerical method for approximating a uniformly elliptic oblique derivative problem in two-dimensional simply-connected domains is proposed. The numerical scheme employs a mixed formulation with piecewise affine functions on curved finite element domains. The direct approximation of the gradient of the solution turns the oblique derivative boundary condition into an oblique direction condition. A priori and a posteriori error estimates as well as numerical computations on uniform and adaptive meshes are provided